Volume 19, No. 6, 2022
Antibacterial Activity Of Secondary Metabolites Of Medicinal Plants
Alexander Pérez-Cordero , Carlos Vásquez Blanco , Donicer E Montes-Vergara
Abstract
The objective of this study was to evaluate the antimicrobial activity of essential oils extracted from leaves of Ocimum basilicum and Cymbopogon citratus against Xanthomonas axonopodis pv. manihotis. Plant tissues sampling was carried out in first quarter of 2015 in the municipality of Los Palmitos, Sucre, Colombia. Obtaining essential oils was performed by hydrodistillation assisted microwave. Microdilution technique was used in ELISA plate 96 wells to determine the bactericidal effect of essential oils, concentrations were prepared to 2 500, 1 250, 625, 312, 156, 78, 39, 19 ppm for C. citratus oil and 10 000, 5 000, 2 500, 1250, 625, 312.5, 156.25, 78 ppm O. basilicum oil. The assay was incubated with 180 rpm agitation, to 30 ° C for 24 hours. MTT (3- [4,5-dimethylthiazol-2-yl] -2,5-diphenyl tetrazolium) was used as indicator solution and the absorbance was measured in an ELISA reader at 492 nm to reveal the inhibition of bacterial growth. With obtained data the minimum inhibitory concentration (MIC) was determined and the percentage of bacterial inhibition of each oil at different concentrations. MIC values of C. citratus and O. basilicum oils were 312.5 ppm and 2500 ppm respectively. The essential oil obtained from C. citratus showed higher antimicrobial activity against Xam strain.
Pages: 252-261
Keywords: microdilution, clevenger, Cymbopogon citratus, O. basilicum.