Volume 19, No. 2, 2022

Identification Of Chromatographical Characteristics Of Complicated Biological Feeds


Mr. Ganesh Babu Loganathan , Ms. Amani Tahsin Yasin

Abstract

Model parameters must be exact in order to employ mechanistic models in chromatography. Cleared cell harvest is particularly difficult feedstock for mechanistic models to simulate. A lack of specimen complex and time to establish all of the desirable criteria is also a big hindrance in the way. Therefore, the research's purpose was to build a process that initiates with complicated feedstock including monoclonal antibodies and uses an automated liquid handling system. It was put to the test by comparing separate data sets with estimates produced by the mechanical typical employing all parameters determined in this work. The method was backed up by a near-perfect match between predicted and actual results. The 200 L bed volume of Robo Columns may therefore be effectively used to regulate isotherm parameters for bigger scale column predictions. Mechanistic chromatographic modelling of complex biological feedstocks can benefit greatly from this approach since it provides a new way of finding crucial model input parameters.


Pages: 3786-3803

Keywords: Chromatography, Biological feeds, Robo columbs, feedstocks, Isocratic

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