Volume 19, No. 4, 2022
Experimental Study For Preparation And Characterization Of A Mucoadhesive Gel As A Vehicle For Intranasal Delivery Of Topical Antibiotic Therapy
Amr S Mohammed , Diaa ElDin M. ElHennawi , Mohamed T El-Tabbakh , and Omnia Elsayed Kilany , Ahmed Rifaat Gardouh
Abstract
Background: Mucoadhesive nasal gel provide a good platform of drug delivery to the nasal mucosa. We aimed to detect the histologic effect of mucoadhesive loaded antibiotic to the nasal mucosa of the rabbits by light microscope and scanning electron microscope to detect the ultrastructure changes. Methods: A total of 36 adult male rabbits divided into 3 groups. Vehicle control group (A): Non-diseased, receives intranasal mucoadhesive gel. group (B): Non-diseased, receives intranasal Neomycin-loaded mucoadhesive gel. group (C): Non-diseased, receives intranasal ciprofloxacin loaded mucoadhesive gel. Part of the nasal mucosa was stained by Toluidine blue for scanning light microscopy. Other part of the Nasal mucosa was immediately fixed in 1.5% gluteraldehyde in phosphate buffered saline (pH 7.4) for 2 h at room temperature, and determination of morphological properties and microscopic ultra-structure was performed. Results: There is statistically insignificant difference of histological changes in inflammatory cells of Ciprofloxacin group also surface mucosa showing deciliation of wide area of the epithelial cells and nasal mucosa showing necrobiosis of numerous epithelial cells. Congestion is significantly higher in Neomycin group (46.1%), than Ciprofloxacin group (7.7%).Conclusion: Antibiotic loaded mucoadhesive gel showed minimal damage with preserved integrity of the nasal mucosa, so it could be considered safe vehicle for delivering topical antibiotics.
Pages: 148-159
Keywords: mucoadhesive gel, nanoparticles, antibiotics, nasal mucosa, histopathology.